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Proteomics in Practice: A Guide to Successful Experimental Design
Reiner Westermeier
(Autor)
·
Tom Naven
(Autor)
·
Hans-Rudolf Höpker
(Autor)
·
Wiley-Blackwell
· Tapa Dura
Proteomics in Practice: A Guide to Successful Experimental Design - Westermeier, Reiner ; Naven, Tom ; Höpker, Hans-Rudolf
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Reseña del libro "Proteomics in Practice: A Guide to Successful Experimental Design"
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
